Heart wga staining
Web30 de ago. de 2024 · To evaluate the effect of protocatechuic acid on cardiomyocyte morphology and cell size, heart tissues were stained with hematoxylin and eosin (H&E) … Web5 de ene. de 2024 · Wheat Germ Agglutinin Staining (WGA) in FFPE tissue sections? Has anybody experience with WGA staining in formalin-fixed and paraffin-embedded tissue …
Heart wga staining
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Web1 de dic. de 2014 · The classic way to do is to stain cardiomyocytes with Rhodamine phalloidin and using any microscopy program measure the are of the stained … Web12 de abr. de 2024 · WGA staining showed increased pathological hypertrophy of cardiomyocytes in the non-infarcted region (Fig. 2H, I). Elevated HF indicators (ANP, BNP, and MyHC) were also observed at the same time (Fig. 2J). All of these changes suggested that 30 days after MI, the mice developed severe cardiac remodeling and even heart …
WebWheat Germ Agglutinin (WGA) is a well-known lectin originally noted for its differential ability to agglutinate cancerous versus normal cells [1] and later identified as a plasma … WebLoss of PTEN promotes cardiac regeneration and protects the heart from myocardial infarction. A, A schematic diagram of the research design and experimental procedures.Four-week-old Pten fl/fl; α-MHC-MerCreMer (Pten cKO) mice, and Pten fl/fl (control) mice were intraperitoneally injected with tamoxifen (75mg/kg body weight). Four …
WebBtg1 expression in the left ventricle in the first postnatal month is highest at P0-P7 (Fig. 1 A, B) coincident with onset of cardiomyocyte cell cycle arrest, and expression is decreased by P15-P30 (Fig. 1 A, green stain).By mRNA quantification of Btg1 by RT-qPCR, higher gene expression of Btg1 is observed in early neonatal hearts, followed by downregulation at … Web17 de dic. de 2014 · The heart sections after SR (left image) and WGA-FITC staining (right image) are shown in Figure 2. The SR staining marks the fibrotic tissue within the …
Web22 de abr. de 2024 · WGA (green), cardiac troponin T (red) and nucleus (blue). e, Quantification of TUNEL + cardiomyocytes in DiKO hearts at one week and two months after deletion. f, Immunostaining for PH3 (green)...
Web4 de abr. de 2024 · Tissue clearing and staining (e.g., with fluorescent dyes, fluorescently labeled antibodies, or nucleic acid probes) are usually conducted with the following steps: (1) fixation, (2) permeabilization, (3) staining (if applicable), (4) bleaching (optional), (5) adjusting of RI using tissue clearing, and (f) RI matching, and imaging. potty training infographicWeb15 de jul. de 2024 · C WGA staining (Green) was used to measure cardiomyocyte size in the heart tissues of TAC mice. D Statistical analysis of the cross section areas of the indicated group. E Representative results of the Masson staining to assess fibrosis of the mouse heart tissues. F Detection of left ventricular end-diastolic diameter (LVEDd) in … tourist in germanWebWheat germ agglutinin (WGA) staining was used to analyse cell size. Results: Increased cytosolic calcium leads to activation of calmodulin-dependent protein kinase II/histone deacetylase 4 (CaMKII/HDAC4) signalling and upregulation of the foetal genes ANP and BNP, indicating cardiac remodelling. potty training incentivesWeb7 de oct. de 2024 · Isolated cardiomyocytes were stained for Troponin T and α-actinin to assess purity of the obtained population. Furthermore, we developed an image analysis platform to determine cardiomyocyte nucleation and ploidy status following DAPI staining. Image based ploidy assessments led to consistent and reproducible results. potty training incentives for boysWeb17 de dic. de 2014 · WGA staining was performed to visualize cellular membranes and indicate cardiac fibrosis (Emde et al., 2014). Finally, slides were washed in PBS and … tourist in hotel roomWeb1 de dic. de 2024 · TUNEL staining was performed to detect the apoptosis of cardiomyocytes. The level of phosphorylated p65 was detected by Western blot. The effect of KLF15 on nuclear factor-κB（NF-κB）transcriptional activity was detected by luciferase reporter assay. RESULTS：KLF15 was down-regulated in hearts at 1 d after MI injury. tourist in halifaxWebGlycogen, diastase-PAS staining of the sarcoplasm, and IgG were also studied and compared with the structure of the lesions and the distribution of myoglobin. The surgical … potty training incentive charts