Phospho- ser/thr pka substrate antibody

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August undefined, 2024

WebPhospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also … WebNov 2, 2024 · Proteins retained on sepharose were incubated with an active recombinant PKA Cα subunit for the kinase assay and then blotted with an anti-phospho- (Ser/Thr) PKA substrate antibody or anti-myc...

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Web3 a 1,2,3 875 879 9621 Phospho-(Ser/Thr) PKA Substrate Antibody 76 3 a 4,5,6 1170 1169 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody 140 3 a 7,8,9 1176 1175 9631 Phospho-(Ser/Thr) Phe Antibody 20 3 a 10,11,12 intentionally left blank 3 b … WebThe inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. simple rules for trading stocks

Phospho-(Ser/Thr) PKA Substrate Antibody

WebPartially purified Xenopus EF-2 (xEF-2) was phosphorylated by PKA in vitro and this phosphorylation was detected by Western blotting using PKA substrate antibody. The phosphorylation of Thr-57 in xEF-2 (corresponding to Thr-56 of the mammalian enzyme) was detected in the partially purified xEF-2 from the resting oocytes, but this xEF-2 did not ... WebPhospho-PKA alpha (Ser338) Polyclonal Antibody detects endogenous levels of PKA alpha only when phosphorylated at Ser338. Target Information Protein kinase A (PKA, cAMP … WebMonoclonal Antibody for studying PKA Substrate motif [RXX(t/s)] phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-(Ser/Thr) PKA Substrate Antibody (CST #5045) is ready to ship. simple runtime windows editor

Phospho-(Ser/Thr) Kinase Substrate - Cell Signaling …

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WebMay 20, 2024 · Epigallocatechin-3-gallate (EGCG) has widespread effects on adipocyte development. However, the molecular mechanisms of EGCG are not fully understood. We investigate the adipogenic differentiation of human-derived mesenchymal stem cells, including lipid deposition and changes in the expression and phosphorylation of key … WebPolyclonal Antibody for studying PKA (RRXt) substrate motif phosphate. Cited in 210 publications. Validated for WB, IP, IHC, E. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-(Ser/Thr) PKA Substrate Antibody (CST … simple rules for playing chessWebdetermining its substrate speciﬁcity, we assayed human recombi-nant AurA on a variety of phosphoacceptor peptide substrates in-cluding a series of properly modiﬁed derivatives of the Kemptide (ALRRASLGAA). The data presented here show that AurA is a basophilic Ser/Thr protein kinase recognizing the consensus raycast definition

"WebPhospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, … " - Phospho- ser/thr pka substrate antibody

Phospho- ser/thr pka substrate antibody

Store at -20°C Serine/Threonine Kinase Substrate Screening Kit

WebImmunogen was a synthetic phospho-PKA substrate peptide. Phospho- (Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine …

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WebMonoclonal Antibody for studying PKA Substrate motif [RXX(t/s)] phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-(Ser/Thr) PKA … WebPhosphoserine/threonine/tyrosine Antibody (61-8300) in ELISA. Direct ELISA analysis of Phosphotyrosine, Phosphoserine, and Phosphothreonine was performed by coating wells of a 96-well plate with 100 µL per well of …

WebDec 15, 2009 · (C) Thr-90-phosphorylation status of Hsp90α in the CM and TCL. pT90-Hsp90α was detected using anti-phospho-(Ser/Thr) PKA substrate antibody. (D) Secretion and Thr-90-phosphorylation status of Hsp90α upon overexpression of Flag-tagged PKA (Left) or upon siRNA treatment (Right) detected by Western blotting and ELISA. PKA was … WebBuy pka antibodies from Santa Cruz Biotechnology, Inc. Monoclonal antibodies are available to most protein immunogens. ... PKA Phosphopeptide Substrate Antibody (15B12) sc-65491: mouse IgG 1: N/A: ELISA: NSR: NEW (1) ... Phospho-Specific PKA Antibodies PRODUCT NAME CATALOG # ISOTYPE EPITOPE APPLICATIONS Species Citations

WebA pan-SIK–specific kinase inhibitor reduces both the hyperphosphostate and the elevated sleep need in Sleepy mutant mice, as well as sleep-deprived WT mice. SIK3 has a serine-threonine kinase domain at the N terminus and a protein kinase A (PKA) recognition site S551 in the middle portion ( 7 ). WebSep 9, 2005 · Western blot using a phospho-Thr antibody of GST-EEA1 (1257–1411) wt or T1392A (1 μg) after phosphorylation with MKK6DD-activated p38α or MKK6DD alone (right panels). ( B ) Endogenous EEA1 was immunoprecipitated from MKK6DD-expressing HEK293 cells (left panel) or SH-SY5Y cells (right panel) and blotted with antibodies against EEA1 …

WebInactivation of a Gαs–PKA tumour suppressor pathway in skin stem cells initiates basal-cell carcinogenesis

WebSee all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species Rabbit Tested applications Suitable for: ELISA, IP, WB, ICC/IF … simple rules for pickleballWebImmunodetection of phospho- (Ser/Thr) PKA substrates shows that phosphorylated proteins are localized differently in spermatozoa of carp and sterlet. Strong phosphorylation of PKC substrate was observed in flagella of sterlet spermatozoa, whereas in carp sperm, PKC substrates were lightly phosphorylated in the midpiece and flagella. raycast dlWebThe Anti-phospho PKA-Eu cryptate is a labeled polyclonal antibody enabling the detection of peptides and proteins containing a phosphorylated Ser/Thr residue with Arginine in the 3 position. It does not detect the non-phosphorylated PKA. This allows the construction of a highly specific biochemical kinase assay. simple rules to play cribbageWebFor antiphospho-PKA substrate (RRXS/T) (100G7E) antibody, a rabbit monoclonal antibody against pPKA substrate was used (commercially available from Cell Signaling). For … simple running shoesWebApr 13, 2024 · In particular, we saw increased phosphorylation of connexin-43 at Ser325/Thr326, and decreased levels of the phospho-peptide, Ser-Thr 320-345 (Figure 4A’; Supplementary Table S4). Notably, the phosphorylation of Ser325 on connexin-43, along with nearby residues Ser328 and Ser330, is mediated by casein kinase 1 and has been shown … simple rugged phonesWebSee all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species Rabbit Tested applications Suitable for: ELISA, IP, WB, ICC/IF more details Species reactivity Reacts with: Species independent Immunogen Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . simple running stitchWebOct 19, 2005 · Four hundred microliters NP40-solubilized sperm lysate (2 mg/ml) was incubated with 4 μl of the Phospho-(Ser/Thr) PKA substrate antibody and 40 μl of 50% protein A-Sepharose CL4B beads. Precipitated immunocomplexes, bound to the protein A beads, were washed four times in NP40 lysis buffer without phosphatase inhibitors and … raycast define