WebbDownload scientific diagram Binding partners of collagen V. (a) Transmission electron microscope (TEM) analysis of suprastructures isolated with superparamagnetic … Webbshape tissue-specific function. The extracellular matrix is mainly composed of glycoproteins, proteoglycans and collagens. SLRPs, with their multivalent binding …
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Webb28 aug. 2011 · The observed G′ of this collagen mimetic system is similar to that typically observed for a collagen hydrogel formed from natural sources, such as rat-tail collagen, … WebbSome SLRPs (asporin, decorin, fibromodulin and lumican) can be associated with collagen fibrils and may be regulatory elements in the formation of collagen-based matrices. Asporin contains a unique aspartic acid (D) repeat … inyectable ampd
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Interaction analysis between OMD (or muteins) and collagen was analyzed using SPR in a Biacore T200 instrument (GE Healthcare). A CM5 Biacore sensor chip (GE Healthcare) was used and activated by a treatment with N-hydroxysuccinimide/N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride, … Visa mer Expression and purification of human full-length OMD and muteins were performed as described previously11. Human OMD (without residues 1–20 corresponding to the signal peptide) with a C-terminal FLAG-tag was inserted into … Visa mer SEC-MALS was performed at room temperature and using PBS buffer (pH 7.4). Proteins concentrated at 25 µM were loaded on a Hiload 10/300 Superdex 200 pg column (GE … Visa mer Data collection was carried out at beamline AR-NE3A at the Photon Factory (Tsukuba, Japan) at 1.000 Å and 100 K. Diffraction images were processed with the program … Visa mer Purified OMD (9.9 mg/ml) was screened in an Oryx8 automatic protein crystallization system (Douglas Instrument) with commercially available … Visa mer WebbDecorin, fibromodulin and lumican are small leucine-rich repeat proteoglycans (SLRPs) which interact with the surface of collagen fibrils. Together with other molecules they … Webb14 juli 2008 · Musculoskeletal disorders that affect the knee and hip represent a major cause of disability and morbidity in Western societies, exert a severe socioeconomic … inyeccion manufactura